A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2014.
Ahead of Print -Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014 - Volume 21, Number 8—August 2015 - Emerging Infectious Disease journal - CDC
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