Genome Detectives Solve Mystery of Hospital’s K. Pneumoniae Outbreak

The following is an excerpt from an article in 

The New York Times
Thursday, August 23, 2012

Genome Detectives Solve Mystery of Hospital’s K. Pneumoniae Outbreak

By GINA KOLATA

The ambulance sped up to the red brick federal research hospital on June 13, 2011, and paramedics rushed a gravely ill 43-year-old woman straight to intensive care. She had a rare lung disease and was gasping for breath. And, just hours before, the hospital learned she had been infected with a deadly strain of bacteria resistant to nearly all antibiotics.

The hospital employed the most stringent and severe form of isolation, but soon the bacterium, Klebsiella pneumoniae, was spreading through the hospital. Seventeen patients got it, and six of them died. Had they been infected by the woman? And, if so, how did the bacteria escape strict controls in one of the nation’s most sophisticated hospitals, the Clinical Center of the National Institutes of Health in Bethesda, Md.?

What followed was a medical detective story that involved the rare use of rapid genetic sequencing to map the entire genome of a bacterium as it spread and to use that information to detect its origins and trace its route.

“We had never done this type of research in real time,” said Julie Segre, the researcher who led the effort.

The results, published online Wednesday in the journal Science Translational Medicine, revealed a totally unexpected chain of transmission and an organism that can lurk undetected for much longer than anyone had known. The method used may eventually revolutionize how hospitals deal with hospital-acquired infections, which contribute to more than 99,000 deaths a year.

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